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KMID : 0923620130130060295
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Immune Network
2013 Volume.13 No. 6 p.295 ~ p.300
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Influence of the Adjuvants and Genetic Background on the Asthma Model Using Recombinant Der f 2 in Mice
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Chang Yoon-Seok
Kim Yoon-Keun
Jeon Seong-Gyu
Kim Sae-Hoon
Kim Sun-Sin
Park Heung-Woo
Min Kyung-Up
Kim You-Young
Cho Sang-Heon
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Abstract
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Der f 2 is the group 2 major allergen of a house dust mite (Dermatophagoides farinae) and its function has been re-cently suggested. To determine the optimal condition of sen-sitization to recombinant Der f 2 (rDer f 2) in murine model of asthma, we compared the effectiveness with different ad-juvants in BALB/c and C57BL/6 mice. Mice from both strains sensitized with rDer f 2 by intraperitoneal injection or subcutaneous injection on days 1 and 14. The dosage was 20?g. Freund’s adjuvants with pertussis toxin (FP) or alum alone were used as adjuvants. On days 28, 29, and 30, mice were challenged intranasally with 0.1% rDer f 2. We eval-uated airway hyperresponsivenss, eosinophil proportion in lung lavage, airway inflammation, and serum allergen specif-ic antibody responses. Naive mice were used as controls. Airway hyperresponsiveness was increased in C57BL/6 with FP, and BALB/c with alum (PC200: 13.5±6.3, 13.2±6.7 vs. ＞50 mg/ml, p＜0.05). The eosinophil proportion was in-creased in all groups; C57BL/6 with FP, BALB/c with FP, C57BL/6 with alum, BALB/c with alum (24.8±3.6, 20.3± 10.3, 11.0±6.9, 5.7±2.8, vs. 0.0±0.0%, p＜0.05). The se-rum allergen specific IgE levels were increased in C57BL/6 with FP or alum (OD: 0.8±1.4, 1.1±0.8, vs. 0.0±0.0). C57BL/6 mice were better responders to rDer f 2 and as for adjuvants, Freund’s adjuvant with pertussis toxin was better.
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KEYWORD
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Allergy, Asthma, Rodent, House dust mite, Der f 2
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